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Read and download RNS 300 Navigation CD Player manuals online (PDF) Get a free RNS 300 Navigation CD Player manual (PDF) Read manuals and view detailed RNS 300 Navigation CD Player, Wiring Diagram, manuals. This is the easiest way to choose a RNS 300 Navigation CD Player. Type in the RNS 300 Navigation CD Player model number in the search bar and click Go!Direct and indirect effects of in vitro pregnancy on mononuclear cells from maternal blood.
Blood mononuclear cells (MNC) of pregnant women are known to be primed and activated, and direct and indirect effects of pregnancy on these cells have been demonstrated. The aim of the present study was to investigate the direct and indirect effects of pregnancy on MNC of the maternal circulation in the peripartum period. Twenty primigravida women with singleton gestation were enrolled into the study. The first measurement was performed at 6-8 wk of gestation, and the second at the end of pregnancy, during the delivery. Controls included 10 women without pregnancy. Flow-cytometric analysis of CD11b expression, intracellular perforin, MHC-class II, and CD86 expression by MNC of the maternal circulation in response to ex vivo IL-2 priming was performed. Pregnancy and the delivery were accompanied by a 5-fold increase in both the total number of CD11b-expressing cells and activated (IL-2-primed CD11b-positive) MNC compared to nonpregnant controls. Increased total numbers of perforin-positive cells were observed in women in the late stage of pregnancy, as well as during the delivery. The number of CD86-positive MNC increased significantly in the second trimester of pregnancy, and decreased at the time of delivery. In addition, both the absolute number of IL-2-primed cells and the absolute number of perforin-positive cells were increased in the late stage of pregnancy and during the delivery. During the delivery there was a significant increase in the number of IL-2-primed cells expressing perforin, in contrast to IL-2-primed cells not expressing perforin in the late stage of pregnancy. These data indicate that the pregnancy process was accompanied by priming, activation, and maturation of the maternal immune system in the peripartum

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